Table 3. Expression level differences of CA metabolism genes between E. coli K92 and E. coli K92ΔrcsA or E. coli K92ΔrcsB measured by qPCR at 19 and 37°C.
| Function | Gene* | Product† | E. coli K92ΔrcsA/ E. coli K92‡ 19°C | E. coli K92ΔrcsB/ E. coli K92‡ 19°C | E. coli K92ΔrcsB/ E. coli K92‡ 37°C |
|---|---|---|---|---|---|
| CA synthesis | wzb | Tyrosine phosphatase | −1.4±0.2 | −16.9±1.7 | −2.1±0.3 |
| wzc | Tyrosine kinase | −1.7±0.2 | −14.7±2.2 | −2.7±0.3 | |
| wcaA | Putative colanic acid glycosyltransferase | −2.7±0.3 | −17.9±2.1 | −2.3±0.4 | |
| wcaD | Colanic acid polymerase | −6.9±0,9 | −23.3±3.1 | −3.1±0.5 | |
| gmd | GDP-mannose 4,6-dehydratase | −8.5±1.3 | −35.7±3.7 | −28.8±4.8 | |
| fcl | GDP-fucose synthase | −7.3±1.5 | −29.7±3.1 | −21.8±5.5 | |
| wcaK | Putative colanic acid piruviltransferase | −6.8±0.8 | −9.2±1.2 | −4.9±1.1 | |
| ugd | UDP-glucose-6-dehydrogenase | +1.4±0.2 | −2.3±0.3 | −3.3±0.2 |
*Genes involved in metabolism of CA.
†Description of the products encoded by genes.
‡The relative levels of gene expression were calculated as described in the Materials and methods, and then transformed to relative change using the formula 2−ΔCT. As a control gene we used the housekeeping gene gapdh.