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Principle of spin (vortex) hydrodynamic focusing integrated in a microfluidic chip. (a) Operation principle with sheath and sample flow directions. (b) Fluorescence image (inverted grey scale) of rhodamine dye 6G used as a sample fluid and excited with mercury arc lamp. To clearly demonstrate the principle of spin focusing the sample flow rate was set to 48 μL/min, above optimal conditions. Ultrapure water was used as sheath fluid with the total flow rate of 1,230 μL/min.
