Table 1.
Primers used in this study. F, forward; R, reverse; h-COL1, collagen type I; h-ALP, alkaline phosphatase; h-OPN, osteoprotein; h-OC, osteocalcin; h-GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
| target gene | direction | 5′–3′ primer sequence |
|---|---|---|
| h-COL1 | F | 5′-CCTGAGCCAGCAGATCGAGAA-3′ |
| R | 5′-GGTACACGCAGGTCTCACCAGT-3′ | |
| h-ALP | F | 5′-TTGACCTCCTCGGAAGACACTC-3′ |
| R | 5′-CCATACAGGATGGCAGTGAAGG-3′ | |
| h-OPN | F | 5′-CTGAACGCGCCTTCTGATTG-3′ |
| R | 5′-ACATCGGAATGCTCATTGCTCT-3′ | |
| h-OC | F | 5′-GGCGCTACCTGTATCAATGGC-3′ |
| R | 5′-TGCCTGGAGAGGAGCAGAACT-3′ | |
| h-GAPDH | F | 5′-CCTGCACCACCAACTGCTTA-3′ |
| R | 5′-AGGCCATGCCAGTGAGCTT-3′ |