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. 2012 Dec 13;(70):4287. doi: 10.3791/4287
Cell type Final Cell Concentration Final Dye Concentration Reference
hPBMCb 1 x 107/ml 2 μM PKH67 10,17
5 x 106/ml 2 μM PKH26 12
3 x 107/ml 10 μM PKH26 13
5 x 107/ml 30 μM PKH26 18
1 x 106/ml 1 μM CellVue Claretc 18
3 x 107/ml 4 μM CellVue Claret 13
5 x 107/ml 5 μM CellVue Claret 18
Cells in culture 5 x 105/ml 0.1 μM PKH26 (1°mammary cells) 8
1 x 107/ml 15 μM PKH26 (U937) 18
1 x 107/ml 12.5 -15 μM PKH26 (U937) 15
1 x 107/ml 1 μM PKH67 (K562) 18
1 x 107/ml 1 μM PKH67 (T cell lines) 9
1 x 107/ml 10 μM CellVue Claret (YAC-1) 23