Cell type | Final Cell Concentration | Final Dye Concentration | Reference |
hPBMCb | 1 x 107/ml | 2 μM PKH67 | 10,17 |
5 x 106/ml | 2 μM PKH26 | 12 | |
3 x 107/ml | 10 μM PKH26 | 13 | |
5 x 107/ml | 30 μM PKH26 | 18 | |
1 x 106/ml | 1 μM CellVue Claretc | 18 | |
3 x 107/ml | 4 μM CellVue Claret | 13 | |
5 x 107/ml | 5 μM CellVue Claret | 18 | |
Cells in culture | 5 x 105/ml | 0.1 μM PKH26 (1°mammary cells) | 8 |
1 x 107/ml | 15 μM PKH26 (U937) | 18 | |
1 x 107/ml | 12.5 -15 μM PKH26 (U937) | 15 | |
1 x 107/ml | 1 μM PKH67 (K562) | 18 | |
1 x 107/ml | 1 μM PKH67 (T cell lines) | 9 | |
1 x 107/ml | 10 μM CellVue Claret (YAC-1) | 23 |