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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Dev Biol. 2013 Feb 4;376(2):213–223. doi: 10.1016/j.ydbio.2013.01.033

Figure 5. TnI Reporter activity requires an intact E-box.

Figure 5

A. Diagram illustrating the location of an essential E-box and its mutated counterpart (both shown in bold) in the two TnI reporter plasmids. Arrow indicates the position of the normal translation start site. B. C. Expression of β-galactosidase in early tail formation stage embryos electroporated with CiTnI500nZ and CiTnI500EboxSDMnZ respectively. Scale bars are 500μm. D. E. Activity of β-galactosidase in cleavage-arrested embryos co-electroporated with CiTnI500nZ and pTCiMRFb. F. Activity of β-galactosidase in a cleavage-arrested embryo co-electroporated with CiTnI500nZ and pSP72. G. Example of an embryo co-electroporated with CiTnI500EboxSDMnZ and pTCiMRFb showing no β-galactosidase activity. Scale bars in D–G are 50μm.