Table 1.

Muscle Genes Assayed

Gene/Gene Family	Abbreviation	Feature	Clone ID*	Accession #
Actin	Actin	Thin Filament	citb095F03	XM_002126220
Myosin Heavy Chain	MHC	Thick Filament	cilv003k12	AK115565.1
Myosin Regulatory Light Chain	MRLC	Thick Filament	citb104p01	AK116716.1
SET-MYND Domain	SMYD-1	Gene Regulatory†	citb009d08	AK112854.1
Tropomyosin 2	TPM2	Thin Filament	cilv034e06	AK174927.1
Troponin I	TnI	Thin Filament	pcTp2	U55261
Acetylcholinesterase	Ache	Cholinergic	NC	NM_001128877
Creatine Kinase	CKM	Metabolic	citb072f10	NW_001955200
Myosin (alkali) Light Chain	MLC	Thick Filament	cilv022011	AK174821.1
Troponin T	TnT	Thin Filament	citb012e12	NW_001955435

^*Clone ID refers to the cDNA clone that was used for preparing probes for in situ hybridization. Transcripts of the six genes in bold type were detected in the notochord when Ci-MRF was expressed in that lineage; transcripts of the four genes in regular type were not detected in the notochord under those conditions.

^†SMYD-1 also appears to play a role in sarcomere assembly (Li et al., 2011). No clone (NC) was used for Ache, which was assayed using a histochemical method. Except for pcTp2 (MacLean et al., 1997) all clones were obtained from the National Institute of Genetics of Japan.