Table 2.
LacZ expression in co-electroporated embryos
| CiTnI500nZ | CiTnI500nZ | CiTnI500EBMnZ | CiTnI500EBMnZ | |
|---|---|---|---|---|
| pTCiMRFb | pSP72 | pTCiMRFb | pSP72 | |
| Exp. 1 | ||||
| # E | 64 | 119 | X | 91 |
| # NoE | 5 (8%) | 22 (18%) | X | 78 (86%) |
| # MisEN | 23 (36%) | 0 (0)% | X | 0 (0%) |
| # EC/E | 4.40±2.42 | 1.75±1.28 | X | 0.14±0.35 |
| Exp. 2 | ||||
| # E | 54 | 92 | 71 | X |
| # NoE | 6 (11%) | 58 (63%) | 70 (99%) | X |
| # MisEN | 20 (37%) | 0 (0%) | 0 (0%) | X |
| # EC/E | 3.50±2.45 | 0.50±0.75 | 0.01±0.12 | X |
| Exp. 3 | ||||
| # E | 69 | X | 118 | X |
| # NoE | 4 (6%) | X | 91 (77%) | X |
| # MisEN | 38 (55%) | X | 0 (0%) | X |
| # EC/E | 6.75±3.86 | X | 0.29±0.60 | X |
Plasmid combinations that were co-electroporated into embryos are shown at the top. Numbers in parenthesis indicate the percentage of embryos in a given category relative to the number of embryos examined. Abbreviations used are: # E (number of embryos examined); # NoE (number of embryos not expressing LacZ); # MisEN (number of embryos expressing LacZ in the notochord); #EC/E (mean number of cells expressing LacZ per embryo), and “X” (not done).