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The effect of LY294002 and L-NAME on TSD medicated serum induced NO production in HUVECs. Cells were treated with 10% blank serum or 10% TSD medicated serum for 24 h in presence of LY294002 or L-NAME or not; the production of NO was determined by measuring the stable end product of NO, NO2−, and NO3−. The data were expressed as mean ± SD, n = 8. *P < 0.05 and **P < 0.01.
