Figure 7.

Effects of 10% TSD medicated serum on expression of p-Akt, total Akt, and p-eNOS in presence of LY294002 and L-NAME. Cells were treated with 10% blank serum or 10% TSD medicated serum for 24 h in presence of LY294002, L-NAME, or both. Cells were lysed, and the expression was determined by western blot with specific primary antibody to p-Akt, total Akt, and p-eNOS; bands were displayed with secondary antibody and ECL system. Data were expressed as mean ± SD, n = 3. *P < 0.05 and **P < 0.01 compared with p-eNOS of 10% blank serum group, ^Δ P < 0.05 and ^ΔΔ P < 0.01 compare with p-Akt of 10% blank serum group.