Figure 8.

Effects of 10% TSD medicated serum on the levels of PIP3. Cells were treated with 10% blank serum or 10% TSD medicated serum for 24 h in presence of LY294002 or not. The levels of PIP3 were analysis by flow cytometry with anti-PIP3 antibody and FITC conjugated secondary antibody. (a) is the representative figure of flow cytometry. (b) is the bar graph of relative intensity of fluorescence in three independent experiments. Data were expressed as mean ± SD. *P < 0.05 compared with 10% blank serum and ^Δ P < 0.05 compared with 10% medicated serum.