Figure 5.

PHF-tau⁻ and neurons with Pattern IIa PHF-tau_AT8 staining. Neurons and dendrites in the CA1 of Patients P14 (A–J) and P13 (K–O), injected with Lucifer yellow, and with a soma free of PHF-tau_AT8 (; A–E) or with PHF-tau_AT8–ir tangles (Pattern IIa; F–O). (A–B, F–G and K–L) Stacks of 18 and 35 images, respectively, obtained after combining the channels acquired separately for Lucifer yellow (green) and PHF-tau_AT8 (red). (C–D, H–J and M–O) Stacks of 26–58 optical confocal sections of the basal dendrites of (A and B) and immunostained (; F–G, K–L) Lucifer yellow-injected pyramidal neurons. These and Lucifer yellow-injected neurons were recovered and reprocessed immunocytochemically using anti-PHF-tau_PHF-1. The neuron was non-immunostained for PHF-tau_PHF-1 (PHF-tau⁻), whereas the neurons were also PHF-tau_PHF-1 (Supplementary Fig. 5). Scale bars: A, F and K = 10 µm; C–E, H–J and M–O = 3 µm.