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. 2012 Dec 3;6(3):486–496. doi: 10.1111/eva.12032

Figure 3.

Figure 3

Multiplex PCR in Stenodus leucichthys, Salvelinus malma malma, and Oncorhynchus tshawytscha.PCR co-amplification of sdY and 18S (positive amplification control) in males and females genomic DNA samples (n = 10 animals/sex) from sheefish (S. leucichthys), dolly varden (S. malma malma) and Chinook salmon (O. tshawytscha). In the sheefish and dolly varden, sdY was strictly detected in males, whereas in Chinook salmon, sdY was detected in all males and also in a single female. The positive control 18S was detected in all PCR reactions.