Table 2.
Quantitative PCR primers
| Gene | Primer | Sequence (5′–3′) | Amplicon size (bp) | Reference |
|---|---|---|---|---|
| Cecropin B | Forward | TGGCAGCCCTGTTGCTGCTG | 133 | Genbank AY189810 (Bartholomay et al. 2003) |
| Reverse | GCCTGCACTCCTGCTGCAAC | |||
| Defensin | Forward | AGTGGATTCGGCGTCAACGA | 102 | Genbank AY191319 (Bartholomay et al. 2003) |
| Reverse | GTTTCGGCACACGCAAACCT | |||
| Gambicin | Forward | CTGTGACGACTGCAGGAGAC | 100 | Genbank XM_001866164 |
| Reverse | AATCCTCGCTGAGCTCTCGT | |||
| Transferrin | Forward | AAGTACTCTCCGAACGACGA | 109 | Genbank XM_001865823 |
| Reverse | CCGAGTACTTGTCCGGGTAG | |||
| NO Synthase | Forward | CGAGAAGGCCCACATCTACG | 126 | Genbank XM_001841984 |
| Reverse | CGACAGCATGTACTTCTCCA | |||
| Esterase-2 | Forward | CCGACGAGCTGTCCTATCTG | 216 | Weill et al. (2000) |
| Reverse | CGTCGTTGGCAATGTTCAG | |||
| G6pdh | Forward | CGCGCACGAGGAAAAGTACG | 131 | Genbank CPU09034 |
| Reverse | GGTTTGCGGTCTTCCCAACC |