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CD titration of Oxytricha nova quadruplex (10 μM/strand) with neomycin. A concentrated solution of neomycin was added to the DNA solution followed by five minute equilibration. The ratios of DNA to ligand are depicted on each graph. The CD scans are an average of two scans. The titrations were conducted in 10 mM sodium cacodylate, 0.5 mM EDTA, 100 mM NaCl at (a) pH 5.5 (b) pH 6.8.
