Table 4.
Thermodynamic Profiles of Neomycin Interaction With a Variety of Nucleic Acids. Buffer 10 mM Sodium Cacodylate, 0.5 mM EDTA, 100 mM NaCl at pH 5.5.
| ΔHwc (kcal/mol) |
Tm0 (°C) |
Tm (°C) |
ΔT (°C) |
n | ΔH(T1) (kcal/mol) |
ΔH(T2) (kcal/mol) |
ΔCp (cal/mol.K) |
KT(20°C) (M−1) |
|
|---|---|---|---|---|---|---|---|---|---|
| poly(dAdT)•poly(dAdT) | 3.90 | 62.4 | 63.2 | 0.8 | 7.3 | −2.1±0.1 (40°C) | −2.3±0.1 (53°C) |
−16±10 | (8.9±0.1)×104 |
| poly(dA)•2poly(dT) | 1.21 | 23.5 | 31.0 | 7.5 | 6.0 | −0.6±0.3 (5°C) | −3.2±0.5 (15°C) |
−264±30 | (2.4±0.1)×105 |
| poly(rA)•poly(dT) | 7.12 | 64.1 | 67.5 | 3.4 | 8.0 | −4.5±0.1 (35°C) | −5.0±0.2 (45°C) |
−50±20 | (3.4±0.4)×106 |
| poly(dA)•poly(rU) | 3.84 | 43.6 | 65.5 | 21.9 | 6.5 | 3.6±0.1 (15°C) | 2.8±0.1 (20°C) |
−160±18 | (9.4±0.1)×106 |
| d(A2G15C15T2)2a | 3.00 | 93.7 | 101.1 | 7.4 | 10.0 | −0.7±0.1 (20°C) | −2.7±0.1 (30°C) |
−173±23 | (1.8±0.6)×107 |
| poly(rA-rA) | 4.05 | 44.0 | 61.5 | 17.5 | 6.5 | −1.8±0.1 (5°C) | −2.1±0.1 (10°C) |
−60±25 | (1.7±0.2)×107 |
| poly(rA)•poly(rU) | 6.34 | 53.4 | 64.7 | 11.3 | 8.0 | 1.7±0.1 (15°C) | −0.7±0.1 (25°C) |
−245±7 | (2.9±0.1)×107 |
| poly(rA)•2poly(rU) | 1.40 | 45.5 | - | - | - | 2.42±0.02(15°C) | 0.02±0.01 (25°C) |
−240±3 | - |
| 16S A-site RNAa | 96.6b | 70.8 | 77.0 | 6.2 | 1.0 | −0.9±0.1 (10°C) | −2.4±0.1 (20°C) |
−151±12 | (8.7±0.9)×107 |
a
Data obtained from ITC titration experiments.
b
The melting enthalpy corresponds to the melting of a molecule of the nucleic acid.