Figure 1.
Concentration dependent UV thermal denaturation profile of HIV TAR RNA with neomycin dimer DPA52. UV thermal denaturation data of HIV-1 TAR RNA at (A) 0, (B) 4 μM (4 mol. eq.) of DPA52. (E) The plot shows the change in thermal stabilization (ΔTm) as a function of molar equivalents of DPA52. Buffer conditions: 100 mM KCl, 10 mM SC, 0.5 mM EDTA, pH 6.8. [HIV TAR RNA] = 1 μM/strand. The heating rate was 0.3 °C.