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. Author manuscript; available in PMC: 2013 Jun 5.
Published in final edited form as: Biochemistry. 2012 Mar 9;51(11):2331–2347. doi: 10.1021/bi201657k

Figure 5.

Figure 5

Salt dependent studies between neomycin dimer (DPA52) and HIV TAR RNA. The binding constants were determined from FID titration using Scatchard plot analysis at (A) 50 mM KCl, (B) 100 mM KCl, and (C) 150 mM KCl. (D) A plot between log (K) as a function of log(KCl) was fit with linear regression and the solid black line reflects the linear fit which results in a slope that reflects the number of ion pairs forming between DPA52 and HIV TAR RNA. Buffer conditions: 10 mM SC, 0.5 mM EDTA, pH 6.8. [HIV TAR RNA] = 200 nM/strand. [EtBr] = 5 μM.