. Author manuscript; available in PMC: 2013 Jun 5.

Published in final edited form as: Biochemistry. 2012 Mar 9;51(11):2331–2347. doi: 10.1021/bi201657k

Table 3.

Representative table of UV thermal denaturation data of ligand / HIV TAR RNA complexes at r_dr = 1 and 2 (r_dr = ratio of neomycin dimer to HIV TAR RNA conc.). Buffer conditions: 100 mM KCl, 10 mM SC, 0.5 mM EDTA, pH 6.8. [HIV TAR RNA] = 1 μM/strand. [Neomycin dimer] = 1 and 2 μM. [Neomycin] = 1 and 2 μM.

Neomycin dimer	T_m	ΔT_{m (at r_dr = 1)}	ΔΔT_{m (r_dr = 2-r_dr=1)}
HIV 1 TAR RNA	68.9	NA	NA
DPA51	79.1	10.2	2.8
DPA52	78.2	9.3	2.5
DPA65	78.2	9.3	2.1
DPA53	78.5	9.6	2.9
DPA54	77.1	8.2	2.2
DPA55	76.4	7.6	2.8
DPA56	74.9	6.1	3.2
DPA58	74.3	5.4	2.9
DPA60	72.1	3.3	3.0
Neomycin	69.1	0.2	0.4