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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Jan 17;1833(5):1222–1234. doi: 10.1016/j.bbamcr.2013.01.007

Figure 1. Identification of stem cell characteristics.

Figure 1

(A) Representative images of morphology, adipogenesis, and osteogenesis of isolated primary ADSCs. Adipogenesis was confirmed with oil droplets, vacuole like structure, and oil red O staining (red color). Osteogenesis was confirmed with Alizarin red staining (red color).(B) Western blot results of AR and α-Tubulin in ADSCs and LNCaP, positive control for AR. qRTPCR was used to quantify the marker expressions of (C) AP2, LPL, and PPARγ for adipogenesis and (D) BSP, Col1, and OPN for osteogenesis in ADSC. Ctrl is vehicle control without differentiation induction. (E) Representative images of morphology, adipogenesis, and osteogenesis for BM-MSCs. (F) Western blot results of AR and α-Tubulin in BM-MSCs and LNCaP. (G) Adipogensis and (H) osteogenesis marker expressions in BM-MSCs. n = 5, *, p-value < 0.05, **, p-value < 0.005 when compared with controls. Student t test was used to perform statistical analysis.