FIGURE 8. Blocking of CR1 in mice inhibits phagocytosis of IC by monocyte/macrophages.
Mice were injected with CR1 blocking antibody (black line) or control antibody (gray line). Five days after injection, splenic CD11b+F4/80+ cells were analyzed for surface expression of CR1 (A) and also were cultured for 30 min in vitro in presence of FITC-labeled OVA-ICs pre-incubated or not with mouse control serum (complement bound ICs) to determine phagocytosis of IC (B). C) Average mean fluorescence intensity (MFI) of FITC-labeled IC in CD11b+F4/80+ cells from control and anti-CR1 injected mice (n=3). ** p < 0.01 when compared to control.