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. 2013 Jun;10(2):147–153. doi: 10.1089/zeb.2012.0811

FIG. 3.

FIG. 3.

LsmNoDesktopSegment is capable of identifying relevant cell types without user input. (A) Maximum intensity projection of dissected, whole-mount, wild-type intestine at the level of the vent labeled with anti-Elavl (green) and anti-5HT (magenta) antibodies. (B) Binary image of all Elavl labeling revealing all enteric neurons shown in A. (C) Binary image of 5HT-positive clusters that do not co-localize with pixels in B, thus revealing only the serotonergic enteroendocrine cells seen in A. (D) Binary image of 5HT-positive clusters that contain pixels from B, which corresponds to serotonergic enteric neurons shown in A. (B–D) Images contain small artifacts that were later ignored during the counting process, allowing only true cells to be counted. Asterisk, nonserotonergic neuron (5HT-, Elavl+); arrowhead, serotonergic neuron (5HT+, Elavl+); arrow, serotonergic enteroendocrine cell (5HT+, Elavl-). Aboral end of the vent is visible at the top of the image. Scale bar=25 μm.