Table 1.

Manual and Computer-Assisted Cell Counts Are Not Statistically Different

	Manually counted cells		LsmCounter output
Intestinal region	Mean	Std. dev.	Mean	Std. dev.	Relative difference	p value
Wild-type mid-intestine
All neurons	102.8	14.7	98.6	14.0	−4.2	0.655
5HT neurons	6.0	2.3	6.4	2.7	0.4	0.809
EE cells	9.6	7.9	8.8	7.2	−0.8	0.871
Wild-type vent
All neurons	65.0	10.0	66.8	10.1	1.8	0.784
5HT neurons	1.6	0.5	1.8	0.8	0.2	0.667
EE cells	10.4	5.3	9.4	4.9	−1.0	0.766

Cell counts were performed on 200 μm lengths of dissected intestine. 5HT-positive, Elavl-negative cells were counted as enteroendocrine cells (EE cells). Relative difference refers to the arithmetic difference between mean cell counts. P values were calculated via two-tailed unpaired Student's t-test. The overall mean relative difference is −0.6 cells. Abbreviations: Std.Dev., standard deviation.