FIG. 6.

Western blot analysis of Akt, S6K1, S6, and 4E-BP1 protein phosphorylation in muscle of fasted (T0) and HPLC- and LPHC-diet 2-h-refed rainbow trout. Twenty micrograms of total protein per lane was loaded on the gel. A representative blot is shown. Graphs represent the ratio between the phosphorylated protein and the total amount of the targeted protein. Results are means±SE (n=6) and were analyzed using one-way ANOVA followed by the Tukey test for multiple comparisons. Mean values for a selected group not sharing a common letter differ (p<0.05).