FIG. 3.

Biliary alterations by Tm and EtOH. (A) Confocal Z-stack projections of liver labeled with anti-BSEP antibody (top panels) and counterstained with DAPI (bottom panels). Canaliculi from control livers present as long, narrow structures. Treatment with Tm and EtOH results in canalicular attenuation and dilation. Bar=10 μm. Numbers underneath each image correspond to the canaliculi:hepatic nuclei counted for 2–4 Z-stacks per treatment. (B) Live images from larvae treated with rhodamine and PED6 to assess bile secretion. Bile secretion was not affected by Tm or EtOH, as noted by the accumulation of PED6 in the gallbladder (arrow, gb). Bar=1 mm.