Table 2.

Rhizobium sp. NT-26 Mutants Isolated on the Basis of a Loss of Motility or Arsenite Oxidation

Mutant	MaGe IDa	Gene	Function	Gene Locationb	Insertionc
Motility
2B5	NT26v4_0222	flgI	Flagellar P-ring protein precursor	221267–222391	258I-O
2D6	NT26v4_0245	flhA	Flagellar biosynthesis protein	243262–245349	38I-O
4H7	NT26v4_0204	fliF	Flagellar M-ring protein	206351–208027	477I-O
5B7	NT26v4_0226	fliP	Flagellar biosynthetic protein	224272–225009	109O-I
6E4	NT26v4_0220	flgG	Flagellar basal-body rod protein	219981–220769	182I-O
10A11	NT26v4_0248		Putative FlgJ-like protein	246658–247212
10G2	NT26v4_2965		Conserved hypothetical protein	2880029–2881285	44O-I
16B6	NT26v4_0245	flhA	Flagellar biosynthesis protein	243262–245349	55I-O
16B7	NT26v4_0240	flgL	Flagellar hook-associated protein	240428–241549	11I-O
18D11	NT26v4_0238	flgE	Flagellar hook protein	237398–238930	363I-O
20E7	NT26v4_0246	fliR	Flagellar biosynthetic protein	245378–246130	110O-I
23E5	NT26v4_0214	flgF	Flagellar basal-body rod protein	216054–216788	44O-I
29G6	NT26v4_0247		Putative FliR/FliJ-like chaperone	246137–246553	91I-O
35A8	NT26v4_2314		Putative two-component sensor histidine kinase	2263060–2264472	113O-I
37C12	NT26v4_2314		Putative two-component sensor histidine kinase	2263060–2264472	48O-I
37H1	NT26v4_2267		Putative ATP-dependent hydrolase protein	2217585–2219546	337O-I
38B4	NT26v4_0206	visR	Master transcriptional regulator of flagellar regulon	209070–209798	53O-I
38G3	NT26v4_0204	fliF	Flagellar M-ring protein	206351–208027	235I-O
39G12	NT26v4_2671		Conserved protein of unknown function	2586119–2587117	94O-I
40E5	NT26v4_0234	fliK	Flagellar hook-length regulator	234324–235835	83I-O
50E11	NT26v4_0250		Conserved integral membrane protein of unknown function	247603–248142	92O-I
61C2	NT26v4_3970		Conserved exported protein of unknown function	3918374–3918853	196I-O
Arsenite oxidation
8G1	NT26v4_p10026	aioX	Putative periplasmic phosphite-binding-like protein precursor; PtxB-like protein	23892–24806	275I-O
11B3	NT26v4_4048	moeB	Putative molybdopterin biosynthesis protein MoeB	3998958–3999725	245O-I
24B7	NT26v4_p10028	aioR	Two-component response regulator	26262–27584	65I-O
37C3	NT26v4_p10026	aioX	Putative periplasmic phosphite-binding-like protein precursor; PtxB-like protein	23892–24806	191I-O
55H7	NT26v4_p10029	aioB	Arsenite oxidase small subunit	27721–28248
60E6	NT26v4_p10030	aioA	Arsenite oxidase large subunit	28261–30798	818I-O

^aIdentification number of the gene in the MaGe interface.

^bPosition of the corresponding gene on the chromosome or the plasmid.

^cPosition of the codon immediately upstream of the transposon insertion site. Subscripts indicate the orientation of the insertion.