FIG. 4.

Changes in islet vasculature during beta cell regeneration. Five-micrometer paraffin sections. Representative sections from each time point are shown. Scale bars=20 μm (a–f) endogenous green fluorescence in vasculature. Cell nuclei (stained with Hoechst) are shown in gray (sections c–f). Beta cells are marked with mCherry red fluorescence (a, e, f) or insulin antibodies (b, c, d). (a) Day 1 control (before Met treatment). (b) A regenerated pancreas from an affected INCFli adult sacrificed at day 14 (after). (c, d) Independent, affected male casper transgenics sacrificed at day 3. (c) Arrow points to pancreatic ducts. An islet containing labeled vasculature is circled. (d) A different affected adult sacrificed at day 3 with labeled beta cells in an islet (dashed circle). Arrow points to a beta cell near labeled vasculature surrounding main pancreatic duct. (e) Affected INCFli adult sacrificed at day 7. Arrow points to region magnified in (f) Dashed circle: islet. (f) Regeneration day 7. Magnification of region in (e). Arrowhead: beta cells emerging from small pancreatic duct. Color images available online at www.liebertpub.com/zeb