Figure 6.

Time course effects of metabolites after treatment with BF and their metabolic pathways in hiHs and hPHs. (A): Supernatants from hiHs and hPHs treated with BF were collected at 24 and 48 hours after treatment, and the amount of metabolite products was determined as analyte peak area and normalized to cell number used. Metabolites identified by MRM transition codes and liquid chromatography RTs (shown in Fig. 5) from each pathway showed time course effects in both hiHs and hPHs. (B): Summary of in vitro metabolic pathways of bufuralol in hiHs and hPHs. The metabolic pathways of drug bufuralol in hiHs were shown to be the same as those in hPHs. Abbreviations: BF, bufuralol; hiH, human induced pluripotent stem cell-derived hepatocyte; hPH, human primary hepatocyte; RT, retention time.