Figure 1.

Neural stem cells (NSCs) isolated from forebrains of neonatal mice express NSC markers and are multipotent. (A–C): Immunocytochemical staining showing expression of intracellular markers nestin (A) and GFAP (B) and surface marker CD15 (C). (D–F): Upon withdrawal of growth factors, NSCs differentiated into three neural lineages, neurons (β-III-tubulin) (D), astrocytes (GFAP) (E), and oligodendrocytes (GalC) (F). 4′,6-Diamidino-2-phenylindole was used for counterstaining. Scale bars = 100 μm (A–C) and 50 μm (D–F). Abbreviations: GalC, galactosylceramidase; GFAP, glial fibrillary acidic protein.