Figure 6.

CD15 enrichment leads to increased oligodendrocyte differentiation at 28 days postinjection (dpi). (A–D): Histograms demonstrating populations of green fluorescent protein-positive and nestin-positive undifferentiated neural stem cells (NSCs) (A), doublecortin-positive and microtubule-associated protein 2a,b-positive neurons (B), glial fibrillary acidic protein-positive astrocytes (C), and APC+ oligodendrocytes (D) at 7 and 28 days post-transplantation. The x-axis depicts unenriched and CD15-enriched populations at 7 and 28 dpi, and the y-axis shows the percentage of different phenotypes in total engrafted cells in the brain. (A): The percentage of nestin-positive cells (undifferentiated NSCs) does not show any significant difference between unenriched and enriched grafts, either at 7 dpi (ns, p > .05) or 28 dpi (ns, p > .05). (B): The percentage of neurons does not show any significant difference between unenriched and enriched grafts both 7 and 28 dpi (ns, p > .05). (C): The percentage of astrocytes does not show any significant difference at 7 and 28 dpi between unenriched (ns, p > .05) and enriched (ns, p > .05). (D): A significant increase in percentage of oligodendrocytes was seen in enriched NSC grafts compared with the unenriched grafts at 28 dpi. ***, p < .001. The percentages are the means ± SEM; n = 3 animals for each group, one-way analysis of variance with Bonferroni correction, p < .05. Abbreviations: ns, not significant; pi, postinjection.