Figure 5.

AT-MSCs secrete higher levels of cytokines compared with BM-MSCs. (A): Cytokine concentrations measured in culture supernatant were higher for AT-MSCs compared with BM-MSCs. A representative experiment is shown from three separate experiments (n = 4 for both groups; IL-6 values represent 1.0 × 10⁻¹ of the measured concentrations; IL-12 values shown are 10× the measured concentrations). (B): IL-6 mRNA expression was also increased in AT-MSCs compared with BM-MSCs (n = 3 for both groups). (C): AT-MSCs showed a slightly enhanced MTT activity; MTT activity of AT-MSCs is shown relative to that of BM-MSCs (n = 3 for both groups). Data are means ± SEM from three different experiments; statistical analysis was performed using Student's t test (*, p < .05; **, p < .01). Abbreviations: AT-MSC, adipose tissue-derived multipotent stromal cells; BM-MSC, bone marrow-derived multipotent stromal cells; IL, interleukin; IP-10, interferon γ-induced protein 10; MCP-1, monocyte chemotactic protein-1; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; TGF-b1, transforming growth factor-β1; VEGF, vascular endothelial growth factor.