Figure 3.

In vitro modeling of the oxygen switch that occurs during transplantation shows that switching neural precursor cells (NPCs) from 20% to 3% O₂ causes significant cell death. (A–C): Representative imaging of calcein and ethidium bromide labeling of the same field of view, after 48 hours of differentiation of day 23 NPCs at 3% O₂, at 20% O₂, or swapped from 20% to 3% O₂. (D): Quantification demonstrated a significant increase in cell death in differentiating NPCs switched from 20% to 3% O₂ compared with those maintained at 3% O₂ throughout (p = .007; n = 4 per group). Scale bars = 50 μm.