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. 2013 May 8;2(6):473–479. doi: 10.5966/sctm.2012-0133

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©AlphaMed Press 1066-5099/2013/$20.00/0

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Figure 2. — IMR90-human induced pluripotent stem cell (hiPSC)-derived dopamine (DA) neurons express dopaminergic and midbrain markers after 14 days of differentiation. (A–F): Immunocytochemical analysis of IMR90-hiPSC-derived DA shows coexpression TH with MAP2 (A), DDC (B), ALDH1A1 (C), VMAT2 (D), calbindin (E), and GIRK2 (F). (G): Quantification of calbindin⁺/TH⁺ and GIRK2⁺/TH⁺ yield in ANL-infected and Ctrl cells. (H): Transcriptional characterization of dopaminergic and midbrain markers in ANL-infected and control IMR90 hiPSC-derived DA neurons. The nuclei are stained with 4′,6-diamidino-2-phenylindole. Scale bar = 40 μm. Student's t test was used. *, p < .05; **, p < .01. Abbreviations: ANL, ASCL1, NURR1, and LMX1A; Ctrl, control; DIV, days in vitro; TH, tyrosine hydroxylase.