Figure 3.

Functional characterization of IMR90-human induced pluripotent stem cell (hiPSC)-derived DA neurons after 21 days of differentiation. (A–C): Immunocytochemical analysis shows that ANL-infected IMR90-hiPSC-derived DA neurons coexpress TH and SYT. (D–G): These cells share electrophysiological activity such as incoming Na⁺ (D) and outgoing K⁺ (E) ionic currents, multiple evoked potential (F), and spontaneous pace-making activity (G). (H): Dopamine content released in the cell culture medium in basal conditions. The nuclei are stained with 4′,6-diamidino-2-phenylindole. Scale bar = 20 μm. Student's t test was used. ***, p < .001. Abbreviations: ANL, ASCL1, NURR1, and LMX1A; Ctrl, control; DA, dopamine; DIV, days in vitro; SYT, synaptotagmin-I; TH, tyrosine hydroxylase.