Table 4.
Microorganisms used in this study
| Strain | Genotype (description) | Reference |
|---|---|---|
| V601 |
Bacteroides uniformis |
This work |
| V923 |
Bacteroides distasonis |
This work |
| V975 |
Bacteroides ovatus |
This work |
| TC2-24 |
Prevotella ruminicola (NRRL # B-50773) |
This work |
| TC27 |
Prevotella ruminicola |
This work |
| D31d |
Prevotella ruminicola |
This work |
| 20-63 |
Prevotella ruminicola |
This work |
| 20-78 |
Prevotella ruminicola |
This work |
| E40a |
Prevotella ruminicola |
This work |
| E42g |
Prevotella ruminicola |
This work |
| H15a |
Prevotella ruminicola |
This work |
| H2b |
Prevotella ruminicola |
This work |
| 118b |
Prevotella ruminicola |
This work |
| 20-92A |
Prevotella ruminicola |
This work |
| CEN.PK2-1C |
S. cerevisiae MATa ura3-52 trp1-289 leu2-3,112 his3Δ1 MAL2-8CSUC2 |
Euroscarf |
| YRH400 |
D5A + integrated (KanMX4; PPGK1-XYL1-TPGK1; PADH1-XYL2-TADH1; PHXT7-XKS1-THXT7) |
[27] |
| YRH561 |
CEN.PK2-1C [pRS414, pRS416] (control strain with empty vectors) |
This work |
| YRH562 |
CEN.PK2-1C [pRH195, pRH218] (low copy XKS1, high copy Piromyces XI) |
This work |
| YRH563 |
CEN.PK2-1C [pRH195, pRH351] (low copy XKS1, high copy B. uniformis XI) |
This work |
| YRH564 |
CEN.PK2-1C [pRH195, pRH352] (low copy XKS1, high copy B. distasonis XI) |
This work |
| YRH565 |
CEN.PK2-1C [pRH195, pRH353] (low copy XKS1, high copy B. ovatus XI) |
This work |
| YRH587 |
CEN.PK2-1C [pRH195, pRH367] (low copy XKS1, high copy P. ruminicola XI) |
This work |
| YRH592 |
CEN.PK2-1C [pRH351, pRH369] (low copy B. uniformis XK, high copy B. uniformis XI) |
This work |
| YRH628 |
CEN.PK2-1C [pRH384] (no XKS1, high copy P. ruminicola XIa) |
This work |
| YRH629 |
CEN.PK2-1C [pRH195, pRH384] (low copy XKS1, high copy P. ruminicola XIa) |
This work |
| YRH630 |
CEN.PK2-1C [pRH385, pRH218] (low copy P. ruminicola XKa, high copy Piromyces XI) |
This work |
| YRH631 |
CEN.PK2-1C [pRH385, pRH384] (low copy P. ruminicola XKa, high copy P. ruminicola XIa) |
This work |
| YRH1114 |
YRH631 adapted for improved D-xylose fermentation |
This work |
| YRH1136 |
YRH1114 with XI and XK vectors (pRH384 and pRH385) evicted |
This work |
| YRH1137 |
YRH114 with XI vector (pRH384) evicted, maintains the P. ruminicola XKa [pRH385] |
This work |
| YRH1138 | YRH114 with XK vector (pRH385) evicted, maintains the P. ruminicola XIa [pRH384] | This work |
a codon adapted for expression in S. cerevisiae.