Table 5.

Plasmids used in this study

Plasmid	Description	Reference
pRS414	pBluescript II SK+, TRP1, CEN6, ARSH4	[39]
pRS416	pBluescript II SK+, URA3, CEN6, ARSH4	[39]
pRS426	pBluescript II SK+, URA3, 2μ origin	[39]
pJ201	Gene synthesis vector	(DNA2.0)
pRH164	pRS414 + PHXT7-MCS-THXT7	[27]
pRH167	pRS426 + PHXT7-MCS-THXT7	[27]
pRH195	pRS414 + PHXT7-S. cerevisiae XKS1-THXT7	[27]
pRH218	pRS426 + PHXT7-Piromyces sp. E2 XI-THXT7	[40]
pRH325	pCR2.1 TOPO + Bacteroides uniformis XI	This work
pRH326	pCR2.1 TOPO + Bacteroides distasonis XI	This work
pRH327	pCR2.1 TOPO + Bacteroides ovatus XI	This work
pRH351	pRS426 + PHXT7-Bacteroides uniformis XI-THXT7	This work
pRH352	pRS426 + PHXT7-Bacteroides distasonis XI-THXT7	This work
pRH353	pRS426 + PHXT7-Bacteroides ovatus XI-THXT7	This work
pRH357	pCR2.1 TOPO + Prevotella ruminicola strain TC2-24 XI	This work
pRH360	pCR2.1 TOPO + Bacteroides uniformis xylB (D-xylulokinase)	This work
pRH367	pRS426 + PHXT7-Prevotella ruminicola XI-THXT7	This work
pRH369	pRS426 + PHXT7-Bacteroides uniformis xylB-THXT7	This work
pRH379	pJ201 + Prevotella ruminicola TC2-24 codon-adapted XI	This work
pRH380	pJ201 + Prevotella ruminicola 23 codon-adapted XK	This work
pRH384	pRS426 + PHXT7-P. ruminicola TC2-24 codon-adapted XI -THXT7	This work
pRH385	pRS414 + PHXT7-P. ruminicola 23 codon-adapted XK -THXT7	This work
pRH544	pRS414 + PHXT7-P. ruminicola 23 codon-adapted XK -THXT7 rescued from YRH1114	This work
pRH545	pRS426 + PHXT7-P ruminicola TC2-24 codon-adapted XI -THXT7 rescued from YRH1114	This work

For all plasmids listed using the HXT7 promoter, P_HXT7 is the truncated version of the HXT7 promoter for constitutive expression.