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. Author manuscript; available in PMC: 2013 Jun 5.
Published in final edited form as: Curr Protoc Hum Genet. 2012 Apr;0 11:Unit–11.1210. doi: 10.1002/0471142905.hg1112s73

Figure 1.

Figure 1

Schematic Illustration of the miRNA library preparation steps. 3’adapter is first ligated to the total RNA (steps 1–5) followed by RT primer annealing (steps 6–7) and 5’adapter ligation (steps 8–10 ). The sample is then revere transcribed (steps 11–12) and PCR enriched using bar-coded primers (steps 13–27) and is then ready for gel extraction of the miRNA library fraction and QC (steps 28–36). The library is now ready for high-throughput sequencing using either a single pass or custom indexing sequencing.