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Final miRNA library analyzed on 2% agarose gel. Following the PCR and clean up steps, half of the final miRNA is loaded on one lane of a 2% E-gel alongside 100 bp (lane 1) and 25 bp ladders (lane 2). The fraction corresponding to miRNA library to be sequenced was extracted on a previous gel by cutting between 125 bp and 175 bp (lane 4) as described in the procedures and also loaded alongside the non-extracted library as a visual reference and expected recovery yield.
