Table 1.
Name | Sequence (5’-3’) |
---|---|
BCmiRNA_3'rApp-adapter | /5rApp/ACGGG’CTAATATTTATCGGTGG/3SpC3/ |
BCmiRNA_5'RNA-adapter | rUrCrCrCrUrArCrArCrGrArCrGrCrUrCrUrUrCrCrGrArUrCrUrC |
BCmiRNA_RT primer | GCTCCACCGATAAATATTAGCCCGT |
BCmiRNA_PCR1 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT |
BCmiRNA_PCR2-BC1 | CAAGCAGAAGACGGCATACGAGATCGATGTGCTCCACCGATAAATATTAGCCCGT |
BCmiRNA_PCR2-BC2 | CAAGCAGAAGACGGCATACGAGATTTAGGCGCTCCACCGATAAATATTAGCCCGT |
BCmiRNA_PCR2-BC3 | CAAGCAGAAGACGGCATACGAGATTGACCAGCTCCACCGATAAATATTAGCCCGT |
BCmiRNA_PCR2-BC4 | CAAGCAGAAGACGGCATACGAGATACGGTGGCTCCACCGATAAATATTAGCCCGT |
BCmiRNA_PCR2-BC5 | CAAGCAGAAGACGGCATACGAGATGCCAATGCTCCACCGATAAATATTAGCCCGT |
BCmiRNA_PCR2-BC6 | CAAGCAGAAGACGGCATACGAGATCAGATCGCTCCACCGATAAATATTAGCCCGT |
BCmiRNA_PCR2-BC7 | CAAGCAGAAGACGGCATACGAGATACTTGAGCTCCACCGATAAATATTAGCCCGT |
BCmiRNA_PCR2-BC8 | CAAGCAGAAGACGGCATACGAGATGATCAGGCTCCACCGATAAATATTAGCCCGT |
BCmiRNA_PCR2-BC9 | CAAGCAGAAGACGGCATACGAGATTAGCTTGCTCCACCGATAAATATTAGCCCGT |
BCmiRNA_PCR2-BC10 | CAAGCAGAAGACGGCATACGAGATGGCTACGCTCCACCGATAAATATTAGCCCGT |
BCmiRNA_PCR2-BC11 | CAAGCAGAAGACGGCATACGAGATCTTGTAGCTCCACCGATAAATATTAGCCCGT |
BCmiRNA_PCR2-BC12 | CAAGCAGAAGACGGCATACGAGATATCACGGCTCCACCGATAAATATTAGCCCGT |
BC_Custom_Indexing | ACGGGCTAATATTTATCGGTGGAGC (optional) |
Notes: All oligonucleotides can be ordered through Integrated DNA Technologies (IDT; http://www.idtdna.com), with HPLC purification. The bar-codes are designed to be read in a single pass read, but a custom indexing primer can also be used if desired. The adenylated adapter can be ordered from IDT, or if on a budget, made as previously described (Vigneault, Sismour, & Church, 2008).