Table 1.

List of Oligonucleotides:

Name	Sequence (5’-3’)
BCmiRNA_3'rApp-adapter	/5rApp/ACGGG’CTAATATTTATCGGTGG/3SpC3/
BCmiRNA_5'RNA-adapter	rUrCrCrCrUrArCrArCrGrArCrGrCrUrCrUrUrCrCrGrArUrCrUrC
BCmiRNA_RT primer	GCTCCACCGATAAATATTAGCCCGT
BCmiRNA_PCR1	AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
BCmiRNA_PCR2-BC1	CAAGCAGAAGACGGCATACGAGATCGATGTGCTCCACCGATAAATATTAGCCCGT
BCmiRNA_PCR2-BC2	CAAGCAGAAGACGGCATACGAGATTTAGGCGCTCCACCGATAAATATTAGCCCGT
BCmiRNA_PCR2-BC3	CAAGCAGAAGACGGCATACGAGATTGACCAGCTCCACCGATAAATATTAGCCCGT
BCmiRNA_PCR2-BC4	CAAGCAGAAGACGGCATACGAGATACGGTGGCTCCACCGATAAATATTAGCCCGT
BCmiRNA_PCR2-BC5	CAAGCAGAAGACGGCATACGAGATGCCAATGCTCCACCGATAAATATTAGCCCGT
BCmiRNA_PCR2-BC6	CAAGCAGAAGACGGCATACGAGATCAGATCGCTCCACCGATAAATATTAGCCCGT
BCmiRNA_PCR2-BC7	CAAGCAGAAGACGGCATACGAGATACTTGAGCTCCACCGATAAATATTAGCCCGT
BCmiRNA_PCR2-BC8	CAAGCAGAAGACGGCATACGAGATGATCAGGCTCCACCGATAAATATTAGCCCGT
BCmiRNA_PCR2-BC9	CAAGCAGAAGACGGCATACGAGATTAGCTTGCTCCACCGATAAATATTAGCCCGT
BCmiRNA_PCR2-BC10	CAAGCAGAAGACGGCATACGAGATGGCTACGCTCCACCGATAAATATTAGCCCGT
BCmiRNA_PCR2-BC11	CAAGCAGAAGACGGCATACGAGATCTTGTAGCTCCACCGATAAATATTAGCCCGT
BCmiRNA_PCR2-BC12	CAAGCAGAAGACGGCATACGAGATATCACGGCTCCACCGATAAATATTAGCCCGT
BC_Custom_Indexing	ACGGGCTAATATTTATCGGTGGAGC (optional)

Notes: All oligonucleotides can be ordered through Integrated DNA Technologies (IDT; http://www.idtdna.com), with HPLC purification. The bar-codes are designed to be read in a single pass read, but a custom indexing primer can also be used if desired. The adenylated adapter can be ordered from IDT, or if on a budget, made as previously described (Vigneault, Sismour, & Church, 2008).