| Component | Volume (µl) | Final Concentration |
|---|---|---|
| 200 ng/µlRNA in dH2O | 5 | 1 µg total |
| 10x T4 RNA Ligase 2tr Buffer | 1 | 1x |
| 10 µM 3’rApp-adapter | 1 | 10 pmoles total |
| 100% DMSO | 1 | 10% |
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