Component | Volume (µl) | Final Concentration |
---|---|---|
200 ng/µlRNA in dH2O | 5 | 1 µg total |
10x T4 RNA Ligase 2tr Buffer | 1 | 1x |
10 µM 3’rApp-adapter | 1 | 10 pmoles total |
100% DMSO | 1 | 10% |
Component | Volume (µl) | Final Concentration |
---|---|---|
200 ng/µlRNA in dH2O | 5 | 1 µg total |
10x T4 RNA Ligase 2tr Buffer | 1 | 1x |
10 µM 3’rApp-adapter | 1 | 10 pmoles total |
100% DMSO | 1 | 10% |