| Component | Volume (µl) | Final Concentration |
|---|---|---|
| 10 mM ATP | 1.5 | 1µM |
| 10 µM 5’ RNA Adapter | 1 | 10 pmoles total |
| T4 RNA Ligase 1 (20 U/µl) | 1.5 | 2 U/µl |
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