| Component | Volume (µl) | Final concentration |
|---|---|---|
| dH2O | 27 | To 50 ul total |
| Reverse Transcribed-miRNAs | 10 | - |
| 5x HF buffer | 10 | 1x |
| 25mM dNTPs | 0.5 | 0.5 mM |
| 25 µM BCmiRNA_PCR1 | 1 | 0.5 µM |
| 25 µM BCmiRNA_PCR2_BC* | 1 | 0.5 µM |
| Phusion DNA pol. (2 U/µl) | 0.5 | 1 U |
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