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The inhibitory effects of the compounds on EGF-induced activation of EGFR. Both 3,4-dihydroxyphenyl acetic acid and (+)-epoxydon inhibited EGF-induced phosphorylation of Tyr1068 in EGFR. HeLa cells were treated with each compound (50 μg/mL for 40 min), followed by exposure to EGF (10 ng/mL for 10 min), except for the negative control (untreated).
