Table 1.
Statistics of four small RNA libraries from soybean
| |
Library namea |
|||
|---|---|---|---|---|
| Leaf+Pi (HPL) | Leaf-Pi (LPL) | Root+Pi (HPR) | Root-Pi (LPR) | |
| Total reads |
22925183 |
26658394 |
21456069 |
28635677 |
| High quality reads |
22643245(100%) |
26377550(100%) |
20957370(100%) |
27944092(100%) |
| Reads smaller than 18nt |
126772(0.56%) |
68893(0.26%) |
347577(1.66%) |
800247(2.86%) |
| Clean reads |
22483373(99.29%) |
26268210(99.59%) |
20564547(98.13%) |
27083979(96.92%) |
| Total small RNA reads mapping to genomeb |
18248364/22483373 (81.16%) |
22176679/26268210 (84.42%) |
20591098/27083979 (76.03%) |
15573680/20564547 (75.73%) |
| Unique small RNA reads mapping to genomeb | 2396442/2901995 (82.58%) | 3301579/3951946 (83.54%) | 2360847/4071174 (57.99%) | 2583592/4641188 (55.67%) |
aFour small RNA libraries were constructed. Total RNA were extracted from soybean leaves and roots grown in +Pi or -Pi nutrient solutions.
Accordingly the small RNA libraries were named leaf+Pi (HPL), leaf-Pi (LPL), root+Pi (HPR), and root-Pi (LPR).
bTotal small RNA reads and unique small RNA reads were mapped to the Glycine max genome v1.0 sequences available in Phytozome ( http://www.phytozome.org).