Table 2.
Conserved and less-conserved miRNAs families detected in four small RNA libraries from soybean
| Family | Size (nt) | Site (5’ to 3’) | Homology in plant species | |||||
|---|---|---|---|---|---|---|---|---|
| |
|
1st |
19th |
A.thaliana |
O.sativa |
Z.mays |
M.truncatula |
P.trichocarpa |
|
Conserveda |
||||||||
| MIR156 |
20-21, 23 |
U (82%) |
A (59%) |
+ |
+ |
+ |
+ |
+ |
| MIR159 |
21 |
U (100%) |
C (100%) |
+ |
+ |
+ |
+ |
+ |
| MIR160 |
21 |
G (100%) |
A (100%) |
+ |
+ |
+ |
+ |
+ |
| MIR162_1 |
21 |
U (100%) |
C (100%) |
+ |
− |
− |
+ |
+ |
| MIR164 |
21 |
U (100%) |
G (100%) |
+ |
+ |
+ |
+ |
+ |
| MIR166 |
20-22 |
U (80%) |
C (50%) |
+ |
+ |
+ |
+ |
+ |
| MIR167_1 |
21-22 |
U (100%) |
C (100%) |
+ |
+ |
+ |
+ |
+ |
| MIR168 |
20-21 |
C (67%) |
A (67%) |
+ |
+ |
+ |
+ |
+ |
| MIR169_2 |
21 |
A (67%) |
C (100%) |
+ |
+ |
+ |
+ |
+ |
| MIR172 |
21 |
U/A/G (33%) |
C (67%) |
+ |
+ |
+ |
+ |
+ |
| MIR390 |
21 |
A (100%) |
G (75%) |
+ |
+ |
+ |
+ |
+ |
| MIR396 |
21 |
G (60%) |
A (60%) |
+ |
+ |
+ |
+ |
+ |
| MIR397 |
21 |
U (100%) |
A (100%) |
+ |
+ |
+ |
− |
+ |
| MIR399 |
21 |
U (100%) |
C (100%) |
+ |
+ |
− |
+ |
+ |
| MIR2118 |
22 |
U (100%) |
C (100%) |
− |
+ |
+ |
+ |
+ |
| MIR408 |
20-21 |
C (67%) |
A (50%) |
+ |
+ |
+ |
+ |
+ |
| MIR482 |
21-22 |
U (56%) |
A (33%) |
− |
− |
− |
− |
+ |
|
Less-conservedb |
||||||||
| MIR1507 |
22 |
U (100%) |
C (100%) |
− |
− |
− |
+ |
− |
| MIR1508 |
21-22 |
U/C (50%) |
U/G (50%) |
− |
− |
− |
− |
− |
| MIR1509 |
21-22 |
U (100%) |
G (100%) |
− |
− |
− |
+ |
− |
| MIR1510 |
21-22 |
A (75%) |
U (50%) |
− |
− |
− |
+ |
− |
| MIR2109 |
21 |
U (100%) |
U (100%) |
− |
− |
− |
− |
− |
| MIR3522 | 22 | U (100%) | U (100%) | − | − | − | − | − |
The miRNAs in four soybean small RNA libraries were classified into conserved and less-conserved miRNAs based on the data in miRBase ( http://www.mirbase.org).
aFor conserved miRNAs, the counterpart of which can be found in other reference plants apart from legume.
bFor less-conserved miRNAs, the homology of which can only be found in legume.
+indicates the existence of miRNA in species, and - shows no homology in plant species.
A, adenosine; C, cytosine; G, guanosine; nt, nucleotide; U, uridine.
A.thaliana, Arabidopsis thaliana; O.sativa, Oryza sativa; Z.mays, Zea mays; M.truncatula, Medicao truncatula; P.P.trichocarpa, Poplus trichocarpa.