Table 5.
Novel soybean miRNAs in four small RNA libraries
|
miRNA |
Sequence (5’ to 3’) |
Size (nt) |
Ch |
Start:End (+/ −) |
Arm |
TPM in HPL |
TPM in LPL |
TPM in HPR |
TPM in LPR |
Registered in |
|---|---|---|---|---|---|---|---|---|---|---|
| miRBase or not | ||||||||||
| miRnov_1a |
AAAGCCATGACTTACACACGC |
21 |
17 |
1401437:1401518: − |
5 |
160 |
173 |
271 |
249 |
No |
| miRnov_1b |
AAAGCCATGACTTACACACGC |
21 |
20 |
223678:223767: − |
5 |
163 |
179 |
281 |
259 |
NO |
| miRnov_2 |
ATTGGGACAATACTTTAGATA |
21 |
18 |
52797184:52797492:+ |
3 |
0.01 |
0.01 |
0.01 |
153 |
NO |
| miRnov_3 |
GGAGATGGGAGGGTCGGTAAAG |
21 |
20 |
35349749:35349874:+ |
5 |
419 |
371 |
351 |
595 |
NO |
| miRnov_4 |
ATATGGACGAAGAGATAGGTAA |
21 |
20 |
40357028:40357130:+ |
5 |
115 |
120 |
249 |
187 |
NO |
| miRnov_5a |
CAGGGGAACAGGCAGAGCATG |
21 |
2 |
837420:837549:+ |
5 |
394 |
307 |
86 |
15 |
NO |
| miRnov_5b |
CAGGGGAACAGGCAGAGCATG |
21 |
10 |
36557001:36557133: − |
5 |
394 |
307 |
86 |
15 |
NO |
| miRnov_6 |
AGAGGTGTATGGAGTGAGAGA |
21 |
13 |
25849778:25849881:+ |
5 |
256 |
122 |
96 |
98 |
NO |
| miRnov_7 |
AGCTGCTCATCTGTTCTCAGG |
21 |
15 |
4318784:4318876:+ |
3 |
48 |
137 |
0.01 |
0.01 |
NO |
| miRnov_8 |
GCTCACTACTCTTTCTGTCGGTT |
21 |
17 |
37759439:37759543:+ |
3 |
609 |
615 |
18 |
24 |
NO |
| miRnov_9 |
TCAATCCTGGAAGAACCGGCG |
21 |
13 |
35514890:35515064:+ |
3 |
44 |
35 |
106 |
51 |
NO |
| miRnov_10 | AGGAAGCTAAGACGGAACTTA | 21 | 14 | 49069088:49069204:+ | 5 | 44 | 0.01 | 110 | 87 | NO |
Soybean novel miRNAs were identified based on Blast analysis of mature miRNA sequences. For novel miRNAs, which were not previously in miRBase and not assigned into any family were denoted by miRnov (soybean-specific).
Ch, chromosome; HPL, leaf+Pi; HPR, root+Pi; LPL, leaf-Pi; LPR, root-Pi; nt, nucleotide; TPM, transcript per million reads; +, sense strand; −, antisense strand.