Table 2.
Hydrolysis of the K4 CPS produced during shake flask experiments
| pH=5 | pH=6 | |||
|---|---|---|---|---|
|
Time |
K4 |
Defructosylated |
K4 |
Defructosylated |
|
(h) |
(mg∙L-1) |
(mg∙L-1) |
(mg∙L-1) |
(mg∙L-1) |
|
3 |
189 |
27 |
199 |
17 |
|
6 |
181 |
35 |
198 |
18 |
| 16 | 162 | 54 | 192 | 24 |
The polysaccharide (216 mg∙L-1) was incubated in the standard growth medium at pH=5 and pH=6 without cells for 16h. The temperature was kept constant at 37°C and agitation was set to 200 rpm. Samples were collected after 3, 6 and 16h of incubation to analyse defructosylation over time.