Table 4.

Sequences of the primers used for constructing strains E.coli K4-pTrc rfaH , EcK4r1 and EcK4r3, and for real time PCR experiments

Primer	Sequence
rfaHpTrc1	5′-TAAAGGAGGTATACAAAAATGCAATCCTGGTATTTACTGTAC-3′
rfaHpTrc2	5′-TTAGAGTTTGCGGAACTCGGTAT-3′
16Sq_Up	5′- GGTGTAGCGGTGAAATGCGTAGAG-3′
16Sq_Dw	5′- CAAGGGCACAACCTCCAAGTC-3′
qkfoC_Up	5′-TTTTCCCTGCCGCACGATCC-3′
qkfoC_Dw	5′-TTCGGTCTGTTGAAGGAGCAATGG-3′
qkfoA_Up	5′-GGATTTAGCGGAAGGGCATGTG-3′
qkfoA_Dw	5′-TTCTGGTGATGACCAACTTTCAGC-3′
P1=pK4EC05_Up	5′-CAGCACAGCAGAGCGAAGTGCATCATATCCTTCCAGATTTAAATTCTTCAAATTAACCCTCACTAAAGGGCG-3′
P2=pk4EC05_Dw	5′-AAACTGTGATCGGGCGTAGGAACCCGCGTAGTCATCGTCGGCGCAGAAGTTTAGAGTTTGCGGAACTCGGTAT-3′
P3=LacZ_Up	5′-CACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAAATTAACCCTCACTAAAGGGCGG-3′
P4=LacZ_Dw	5′- AAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTTAGAGTTTGCGGAACTCGGTATTC-3′
P5=FRT_Dw	5′-TAATACGACTCACTATAGGGCT-3′
P6=pGapA_Up	5′-CCCTCGAGGGCCTTTAAAATTCGGGGCGCCGA-3′
P7=pGapA_Dw	5′-CGGGATCCCGATATTCCACCAGCTATTTGTTAG-3′
P8=rfaH_Up	5′-CGGGATCCCGATGCAATCCTGGATTTTACTGTAC-3′