Table 4.

Numbers and percentages of Douglas-fir sequences with matches to sequences in three protein databases*

	Isogroups (25,002)†				Singletons (102,623)§
	Isogroups with 1 isotig (I1 = 18,774)		Isogroups with >1 isotig (IM = 6228)		Singletons (S = 102,623)
Database	Number	Percent	Number	Percent	Number	Percent
Uniref50	15,054	80.2	3446	55.3	25,757	25.1
TAIR10	13,749	73.2	3260	52.3	15,907	15.5
Annot8r	11,733	62.5	2862	46.0	14,836	14.5

^*Matches were recorded for isogroups and singletons at a tBLASTX E-value < 10^-5.

^†Isogroups are Newbler v2.3 isogroups. For the isogroups with more than 1 isotig (IM subset), a hit was counted only if all isotigs matched the same protein in the database.

^§Singletons are 454 reads that did not assemble with any other reads.