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. 2013 Jun 5;8(6):e65389. doi: 10.1371/journal.pone.0065389

Figure 2. Upregulation of oxidative stress-related genes in DBA retinas with age and IOP.

Figure 2

Bar charts demonstrating oxidative stress-related gene expression in C57 and DBA retinas by qPCR. Gfap decreased 40% in C57 retinas (p>0.67) and increased 47% in DBA retinas (p>0.10) with age. Overall Gfap levels were increased 330% to 1812% in DBA retinas when compared to age-matched C57 retinas (*p<0.001). Cp decreased with age in C57 mice (64.5%; p>0.12) while increasing 183% in DBA mice († p<0.001), which resulted in increased Cp expression in DBA retinas at 7 months (422%) and 10 months (1172%) compared to age-matched C57 mice (*p<0.004). Ho-1 did not change in DBA retinas with age (p>0.65), but decreased 67% in C57 mice (†p>0.009). Compared to C57 retinas, Ho-1 levels increased 184% at 7 months and 625% at 10 months of age in DBA retinas (*p<0.001). Nos-2 decreased with age in C57 retinas (63%; †p<0.003) and increased DBA retinas at 7 months (22%; p>0.2) prior to decreasing at 10 months (59%; §p>0.003). Nos-2 levels were similar in C57 and DBA retinas at 3 months (p = 0.51) and 10 months (p = 0.52), but were increased 341% at 7 months of age (*p<0.001). (Inset) IOP of C57 (average over all ages) and DBA mice used for qPCR (n = 5). Intraocular pressure increased 52% on average in DBA mice with age (*p<0.001). IOP was lower (8%) in 3 month DBA mice compared to C57 mice (p = 0.016); however IOP was elevated 37% by 7 months of age and 44% by 10 months of age in DBA mice compared to C57 mice (*p<0.001).